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The Maynard Group creates new polymeric materials to address unmet needs in medicine, while simultaneously educating the next generation of scientists.

 

 

Latest Publication
Degradable PEGylated Protein Conjugates Utilizing RAFT Polymerization

Decker, C. G.; Maynard, H. D., "Degradable PEGylated Protein Conjugates Utilizing RAFT Polymerization," European Polymer Journal, 2015 DOI: 10.1016/j.eurpolymj.2015.01.025. [LINK]

Poly(ethylene glycol) (PEG)-protein therapeutics exhibit enhanced pharmacokinetics, but have drawbacks including decreased protein activities and polymer accumulation in the body.  Therefore a major aim for second-generation polymer therapeutics is to introduce degradability into the backbone.  In this paper we describe the synthesis of poly(poly(ethylene glycol methyl ether methacrylate)) (pPEGMA) degradable polymers with protein-reactive end-groups via reversible addition-fragmentation chain transfer (RAFT) polymerization, and the subsequent covalent attachment to lysozyme through a reducible disulfide linkage. RAFT copolymerization of cyclic ketene acetal (CKA) monomer 5,6-benzo-2-methylene-1,3-dioxepane (BMDO) with PEGMA yielded two polymers with number-average molecular weight (Mn) (GPC) of 10.9 and 20.9 kDa and molecular weight dispersities (Đ)of1.34 and 1.71, respectively. Hydrolytic degradation of the polymers and reversible covalent attachment of these polymers to lysozyme was analyzed.  Following cleavage of the polymer, an increase in activity was observed for both conjugates, with the released protein having full activity.  The research described in this paper represents a method to prepare PEGylated proteins, where the polymer is readily cleaved from the protein and the main chain of the polymer is degradable. 

 

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Recent News

  • January 2015:Yuta's paper receives cover of Polymer Chemistry and is selected as paper of the week [LINK]
  • October 2014: Congratulations to recent PhDs, En-Wei Lin and Thi Kathy Nguyen!  They will be going to Nalco as a Principal Chemist and UCLA, respectively
  • September 2014: En-wei and Natalie's paper makes Editor's Choice [LINK]
  • September 2014: Congratulations to Muhammet Kahveci who finished his postdoc and moved to Ylidiz Technical University in Turkey as an Assistant Professor of Bioengineering!
  • July 2014: Heather listed in C&EN as Who's who (talks to go to) for the San Francisco National Meeting
  • June 2014: Congratulations to recent PhDs Juneyoung Lee and Nic Matsumoto! They will be going on to postdocs with Bob Grubbs at Caltech and Bert Meijer at Eindhoven, respectively
  • June 2014: Erhan's collaborative paper with Imperial College London receives cover of Adv. Funct. Mater

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Last Updated: September 19, 2014