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The Maynard Group creates new polymeric materials to address unmet needs in medicine, while simultaneously educating the next generation of scientists.

 

 

Latest Publication
Homodimeric Protein-Polymer Conjugates via the Tetrazine-trans-Cyclooctene Ligation

Lorenzo, M. M.; Decker, C. D.; Kahveci, M. U.; Paluck, S. J.; Maynard, H. D.* Homodimeric Protein-Polymer Conjugates via the Tetrazine-trans-Cyclooctene Ligation,” Macromolecules201649, 30-37. [LINK]

Tetrazine end-functionalized telechelic polymers were synthesized by controlled radical polymerization (CRP) and employed to generate T4 Lysozyme homodimers. Mutant T4 Lysozyme (V131C), containing a single surface-exposed cysteine, was modified with a protein-reactive trans-cyclooctene (T4L-TCO). Reversible addition-fragmentation chain transfer (RAFT) polymerization yielded poly(N-isopropylacrylamide) (pNIPAAm) with a number average molecular weight (Mn by 1H-NMR) of 2.0 kDa and a dispersity (Đ by GPC) of 1.05. pNIPAAm was then modified at both ends by post-polymerization with 6-methyl tetrazine. For comparison, 2.0 kDa bis-tetrazine poly(ethylene glycol) (PEG) and 2.0 kDa bis-maleimide pNIPAAm were synthesized. Ligation of T4L-TCO to bis-tetrazine pNIPAAm or bis-tetrazine PEG resulted in protein homodimer in 38% yield and 37% yield, respectively, after only 1 hour, whereas bis-maleimide pNIPAAm resulted in only 5% yield of dimer after 24 h. This work illustrates the advantage of employing tetrazine ligation over maleimide thiol-ene chemistry for the synthesis of protein homodimer conjugates.

 

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Recent News

  • February 2016: Congratulations to Natalie for winning a best poster award at the International Nanobiotechnology meeting!
  • January 2016: Congratulations Emma on being awarded the Foote Fellowship!
  • January 2016: Uland successfully defends his thesis. Congratulations Dr. Lau!
  • December 2015: Priera wins the Edwin W. Pauley Foundation Award!!
  • December 2015: Graduate student Kathryn and Postdoc Liang (PhD Warwick from O'Reilly and Dove) join group.
  • October 2015: Heather's News and Views article in Nature is published [LINK] and Heather is quoted in C&E news. [LINK]
  • October 2015: New students join the group - welcome Priera, Kyle, and Monica.
  • June 2015: Heather appears in video to let undergraduates know what it is like to be a professor. [LINK]
  • May 2015: Yang finishes her postdoc and moves to be an Assistant Professor at Chapman University
  • May 2015: Heather is selected for the Defense Science Study Group for 2016-2017
  • March 2015: Heather named Associate Director of Technology and Development of the CNSI

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Last Updated: September 19, 2014