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The Maynard Group creates new polymeric materials to address unmet needs in medicine, while simultaneously educating the next generation of scientists.

 

 

Latest Publication
Protein-Polymer Conjugation via Ligand Affinity and Photoactivation of Glutathione S-Transferase

Lin, E.-W.; Boehnke, N.; Maynard, H. D. "Protein−Polymer Conjugation via Ligand Affinity and Photoactivation of Glutathione S‐Transferase" Bioconjugate Chem. 2014, 25, 1902-1909.* [LINK]
*
ACS Editor's choice

A photoactivated, site-selective conjugation of poly(ethylene glycol) (PEG) to the glutathione (GSH) binding pocket of glutathione S-transferase (GST) is described. To achieve this, a GSH analogue (GSH-BP) was designed and chemically synthesized with three functionalities: (1) the binding affinity of GSH to GST, (2) a free thiol for polymer functionalization, and (3) a photoreactive benzophe- none (BP) component. Different molecular weights (2 kDa, 5 kDa, and 20 kDa) of GSH-BP modified PEGs (GSBP-PEGs) were synthesized and showed conjugation efficiencies between 52% and 76% to GST. Diazirine (DA) PEG were also prepared but gave conjugation yields lower than for GSBP-PEGs. PEGs with different end-groups were also synthesized to validate the importance of each component in the end group design. End- groups included glutathione (GS-PEG) and benzophenone (BP-PEG). Results showed that both GSH and BP were crucial for successful conjugation to GST. In addition, conjugations of 5 kDa GSBP-PEG to different proteins were investigated, including bovine serum albumin (BSA), lysozyme (Lyz), ubiquitin (Ubq), and GST-fused ubiquitin (GST-Ubq) to ensure specific binding to GST. By combining noncovalent and covalent interactions, we have developed a new phototriggered protein−polymer conjugation method that is generally applicable to GST-fusion proteins.

 

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Recent News

  • October 2014: Congratulations to recent PhDs, En-Wei Lin and Thi Kathy Nguyen!  They will be going to Nalco as a Principal Chemist and UCLA, respectively
  • September 2014: En-wei and Natalie's paper makes Editor's Choice [LINK]
  • September 2014: Congratulations to Muhammet Kahveci who finished his postdoc and moved to Ylidiz Technical University in Turkey as an Assistant Professor of Bioengineering!
  • July 2014: Heather listed in C&EN as Who's who (talks to go to) for the San Francisco National Meeting
  • June 2014: Congratulations to recent PhDs Juneyoung Lee and Nic Matsumoto! They will be going on to postdocs with Bob Grubbs at Caltech and Bert Meijer at Eindhoven, respectively
  • June 2014: Erhan's collaborative paper with Imperial College London receives cover of Adv. Funct. Mater
  • April 2014: Heather featured on UCLA OIP [LINK]

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Last Updated: September 19, 2014