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The Maynard Group creates new polymeric materials to address unmet needs in medicine, while simultaneously educating the next generation of scientists.

 

 

Latest Publication
Trehalose hydrogels for stabilization of enzymes to heat

Lee, J.; Ko, J. H.; Lin, E.-W.; Wallace, P.; Ruch, F.; Maynard, H. D., "Trehalose hydrogels for stabilization of enzymes to heat," Polymer Chemistry, 2015, DOI:10.1039/C5PY00121H. [LINK]

Enzymes can catalyze various reactions with high selectivity and are involved in many important biological processes. However, the general instability of enzymes against high temperature often limits their application. To address this, we synthesized a trehalose-based hydrogel in two steps from commercial starting materials with minimal purification procedures. Mono- and multi-functional trehalose monomers were cross-linked by redox-initiated radical polymerization to form a hydrogel. Phytase, an important enzyme utilized in animal feedstock, was employed to study the effectiveness of the trehalose hydrogel to stabilize proteins against heat. Addition of the phytase solution to the hydrogel resulted in enzyme internalization as confirmed by confocal microscopy. The phytase in the hydrogel retained 100% activity upon heating at 90 °C compared to 39% when the hydrogel was absent. The enzyme could also be recovered from the hydrogel. The trehalose hydrogel synthesis reported herein should be readily scalable for thermal stabilization of a wide variety of enzymes.

 

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Recent News

  • March 2015: Heather named Associate Director of Technology and Development of the CNSI
  • January 2015:Yuta's paper receives cover of Polymer Chemistry and is selected as paper of the week [LINK]
  • October 2014: Congratulations to recent PhDs, En-Wei Lin and Thi Kathy Nguyen!  They will be going to Nalco as a Principal Chemist and UCLA, respectively
  • September 2014: En-wei and Natalie's paper makes Editor's Choice [LINK]
  • September 2014: Congratulations to Muhammet Kahveci who finished his postdoc and moved to Ylidiz Technical University in Turkey as an Assistant Professor of Bioengineering!
  • July 2014: Heather listed in C&EN as Who's who (talks to go to) for the San Francisco National Meeting
  • June 2014: Congratulations to recent PhDs Juneyoung Lee and Nic Matsumoto! They will be going on to postdocs with Bob Grubbs at Caltech and Bert Meijer at Eindhoven, respectively

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Last Updated: September 19, 2014