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The Maynard Group creates new polymeric materials to address unmet needs in medicine, while simultaneously educating the next generation of scientists.

 

 

Latest Publication
Encapsulated Hydrogels by E-Beam Lithography and Their Use in Enzyme Cascade Reactions

Mancini, R. J.; Paluck, S. J.; Bat, E.; Maynard, H. D. “Encapsulated Hydrogels by E-Beam Lithography and Their Use in Enzyme Cascade Reactions,” Langmuir2016, published online. [LINK]

Electron beam (e-beam) lithography was employed to prepare one protein encapsulated inside another by first fabricating protein-reactive hydrogels of orthogonal reactivity and subsequently conjugating the biomolecules. Exposure of thin films of eight arm star poly(ethylene glycol) (PEG) functionalized with biotin (Biotin-PEG), alkyne (Alkyne-PEG) or aminooxy (AO-PEG) end-groups to e-beam radiation resulted in cross-linked hydrogels with the respective functionality. It was determined via confocal microscopy that a nominal size exclusion effect exists for streptavidin immobilized on Biotin-PEG hydrogels of feature sizes ranging from 5 to 40 µm.  AO-PEG was subsequently patterned as an encapsulated core inside a contiguous outer shell of Biotin-PEG. Similarly, Alkyne-PEG was patterned as a core inside an AO-PEG shell. The hydrogel reactive end-groups were conjugated to dyes or proteins of complimentary reactivity, and the three dimensional (3-D) spatial orientation was determined for both configurations using confocal microscopy. The enzyme glucose oxidase (GOX) was immobilized in the core of the encapsulated Alkyne-PEG core/ AO-PEG shell architecture, and horseradish peroxidase (HRP) was conjugated to the shell periphery. Bioactivity for the HRP-GOX enzyme pair was observed in this encapsulated configuration by demonstrating that the enzyme pair was capable of enzyme cascade reactions.

 

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Recent News

  • March 2016:Priera is selected for the NSF Graduate Research Fellowship (GRFP)! Congratulations Priera!
  • February 2016: Congratulations to Natalie for winning a best poster award at the International Nanobiotechnology meeting!
  • January 2016: Congratulations Emma on being awarded the Foote Fellowship!
  • January 2016: Uland successfully defends his thesis. Congratulations Dr. Lau!
  • December 2015: Priera wins the Edwin W. Pauley Foundation Award!!
  • December 2015: Graduate student Kathryn and Postdoc Liang (PhD Warwick from O'Reilly and Dove) join group.
  • October 2015: Heather's News and Views article in Nature is published [LINK] and Heather is quoted in C&E news. [LINK]
  • October 2015: New students join the group - welcome Priera, Kyle, and Monica.
  • June 2015: Heather appears in video to let undergraduates know what it is like to be a professor. [LINK]
  • May 2015: Yang finishes her postdoc and moves to be an Assistant Professor at Chapman University
  • May 2015: Heather is selected for the Defense Science Study Group for 2016-2017
  • March 2015: Heather named Associate Director of Technology and Development of the CNSI

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Last Updated: September 19, 2014