Current Proteins of Interest

• Ferritin
• Heme Oxygenase
• S-Adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase

Methods

The cDNAs have all been assembled from previously sequenced ESTs. The ESTs were selected by finding a gene within the Arabidopsis thaliana genome within GenBank and then searching for similar sequences within the Chlamydomonas reinhardtii genome using a BLAST search. Those ESTs with an Expect value of < 5.0e-5 were then assembled into a single contiguous sequences using DNA* SeqMan for Macintosh. Open reading frames were identified by translation(DNA Strider v.1.2). The translated sequences were then queried against GenBank sequences to ensure similarity to known proteins. These contiguous sequences may not be the actual sequence but are an estimate based on available information.