The preceding experimental work, as explained in the beginning, was mainly performed on polymerizable phospholipids. The study of polymerizable vesicles, which had already led to the discovery of the so-called wrinkling transition in our laboratory, was continued using other kinds of polymerizable phospholipids. Wrinkling transition do not systematically occur, and other behaviors have been observed, like a rigidification on the membrane on polymerization, using a phospholipid we have synthesized. A wide variety of morphological transitions are thus to be expected in the near future, which could shed light on the complicated mechanics of the real cellular wall.
Preliminary work on tubules led us to the conclusion that a local analysis of the wrinkled membrane might be performed using atomic force microscopy (AFM). Comparison of the local topography of the membrane with the prediction of the statistical physics of membrane could then be done at an unprecedented scale.
Finally, we studied the influence of ferrofluids on the equilibrium shapes of vesicles. Very interesting, though unpublished observations have been made, on the dynamical evolution of vesicles whose membranes are covered with ferrofluidic particles, destabilized by a brief and intense magnetic field. A model for the study of cell crawling ?
|page realised by Xavier Michalet||last revised: september 16th,1997|