I've had some interesting questions this week. Everyone has biochemistry on the brain, in anticipation of this week's midterm.

--In a saccharide, if the anomeric carbon is linked in a glycosidic bond, the sugar is considered non-reducing. So, in a polysaccharide where all of the anomeric carbons are involved in glycosidic bonds, each residue is non-reducing. From this, you could say that there are as many non-reducing sugars as there are monomers. However, only the non-reducing residue at the end of each branch is used in the technical definition of the non-reducing end (NRE).

--Lysine has an NH3 group in its sidechain that is susceptible to modification in the N-terminal identification methods used in protein sequencing. There are other amino acids with nitrogens in their sidechains (arg and his) that do not react with N-terminal identification reagents. Why?

--Try writing fractions like this: 1/134 = 1/ (1.34 X 10 -2)
So, you can easily divide 1 by 1.34 (think, 13 into 1) and then just move 10 2 up to the numerator.


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