Merchant Lab Research Resources
 
Merchant Lab Research Resources
Antibodies
- Antibodies vs 27March08.xls - updated March 2008 (requires password
)
Archived Protocols
- Chlamy Chloroplast DNA Protocol
- Chloroplast DNA Prep Protocol
- DNA Preparation from Chlamydomonas Reinhardtii
- Pronase Method for Making DNA From C.r.  
Biosciences Core Facilities at UCLA
Chemical Inventory - see computer in 5069

Departmental Safety Policy
- Chair's Memo on Safety (February 8th, 2009)

- EH&S Online Safety Training
- Radiation Safety Protocols and Procedures (requires password)

Digital Imagery
- Digital Expert Web Site (Resources & Tutorials)
- Preparing Digital Images for Publication in Scientific Journals

Enzymes
- Alphabetical List of NEB Enzymes
- Cleavage Near End of Linear DNA
- Double Digests Buffer Compatibility
- Heat Inactivation
- NEBcutter
- Restriction Enzymes Available

- Setting up a Reaction
- Survival in a Reaction

Facilities Management - report a UCLA facilities problem

Freezer Contents
- Inventory for Chest Freezer - November 2007
- Template for Inventory

Group Meetings Schedule - updated June 2009

Journal Club Papers
- Leichert and Jakob, 2004
- Yamasaki et al. 2007
- Wilson et al. 2007

- Peiter et al. 2007

- Surzycki et al. 2007
- Yoshimoto et al. 2007
- Shao et al. 2007
- Quan et al. 2007

Journal Club Suggestions
-
Hicks et al. 2007
- Ojha and Hatfull, 2007
- Gould et al. 2007
- Miras et al. 2007
- Kimata et al. 2007
- Kobayashi et al. 2007
- Meyer et al. 2007
- Havaux et al. 2007
- Cheng et al. 2007
- Wang et al. 2007
- Wiedemann et al. 2007
- Narsai et al. 2007
- Mukherjee et al. 2007
- Bihlmaier et al. 2007

- Rust, et al. 2007
- Ishihara et al. 2007
- Lee et al. 2007
- Chae et al. 2007
- Shimada et al. 2007
- Delneri et al. 2007
- Camargo et al. 2007
- Eveland et al. 2007
- Mills et al. 2007
- DalCorso et al. 2007
- Wagner et al. 2007
- Ridge et al. 2008
- Imlay 2008
- Regulski et al. 2008
- Horan et al. 2008

Articles of Interest
- Mineral Nutrition from Lambers et al. (requires password)
  
  Large file: To download Mineral Nutrition right click on link and select "Save Target As" (PC) or press Ctrl + Click and select "Download Linked File" (Mac)
- HHMI Making the Right Moves: A Practical Guide to Scientific Management for Post Docs and New Faculty
- HHMI Training Scientists to Make the Right Moves

Lab Manual
- Add database to Mascot server
- Agencourt Instructions Generic
- arg2 vs. arg7 - Courtesy of Lib Harris
- Amino acid analysis at UC Davis
- Brij lysis plasmid prep purified by CsCl gradients
- Cell and size counts by Coulter counter
- Chlamy growth medium
- Chlamy strains storage protocol by liquid N2 freezing (from Paris group via Olivier)
- Chlamydomonas DNA Midiprep
- Chlorophyll assay
     - Porra et al 2002
     - Porra et al 1989

- Chlorophyll Measurement of Cells in a Culture
- CW Determination
- Deb Berthold's suggestions for protein expression in E. coli
- Dynamics of Microbial Growth
    Large file: To download Dynamics of Microbial Growth right click on link & select "Save Target As" (PC) or press Ctrl + Click & select "Download Linked File" (Mac)
- EMBO Course Protocols
- Endnote X1
- Formaldehyde Gel Electrophoresis of RNA & RNA Blot Hybridization
- Freezing protocol (from Umen Lab)
- Home-made ECL Reagents
- Instructions to send samples to Niyogi lab for pigment analysis
- Insurance for Work Related Travel
- Lowry Assay
- Metal content of chlamy cells and chlamy medium
- Methods of Possible Interest
    - Chl and carotenoids
- Native Gel "Angel" Stock Solutions
- Negative Protein Stain
- Oligolabelling Procedure
    - Info on oligolabelling
    - Optimization
    - Optimization - template concentration & size
    - Protocol
    -
Radiochemical Degradation of Nucleic Acids by 32-Phosphorus
    - Schematic
- Operations Manuals
    - O2 Electrode Preparation and Maintenance
- Optimum use of NBS Universal Platform
- pJD100
    - pJD100 Complete Text
    - pJD100GW Complete Text
    - pJD100 Sequence Information
- Preparation of Dialysis Tubing
- Preparation of Iron-Deficient Medium
- Product Technical Information
   -Promega Online
- Purification of Taq (requires password)
- Pipette Washing Instructions
- Radiation Safety Protocols and Procedures (requires password)
- Rapid Isolation of Plasmid DNA - Midi Prep
- Real Time PCR Protocols
    - Eleven Golden Rules of Quantitative RT-PCR
    - Merchant Lab qPCR Protocol (requires password)
    - The PCR Song
- RNA isolation from Chlamydomonas
- Significant Figures
- Springer Protocols
- TAP medium preparation from SM files (original from Togasaki)
- Trypsin in gel digestion

- What is Microbiology?
    Large file
: To download What is Microbiology? right click on link & select "Save Target As" (PC) or press Ctrl + Click & select "Download Linked File" (Mac)
- Zygote DNA Prep (from Jim Umen)

Lab Responsibilities - updated July 2007
Molecular Weight Markers (requires password)
 - Protein ladders
 - DNA ladders
 - RNA ladders
Homologies, Orthologs and Paralogs
- Orthologs and paralogs - we need to get it right
- Homology - a personal view on some of the problems
- “Homology” in proteins and nucleic acids: A terminology muddle and a way out of it
- Phylogeny for the faint of heart: a tutorial
Online Courses
- Animal Subjects
- EH&S Online Safety Training
- Sexual Harrassmment
- Workplace Ethics

Personnel (requires password)

Places to Recycle
Purchase Order List

Replicates & Statistics
- Basic Statistical Considerations in Virological Experiments
- How Robust is Your Data? (Nature Cell Biology)
    - Additional Guidelines (see further editorial information)
- Error Bars
- Experimental Design for Yeast Genomic Expression Analyses

- Sample Size
- Statistical analysis of real time PCR data
- The Power of Replicates

Research Projects
Room Reservation Calendar
Strain List
- Bacteria
- Yeast
- Chlamydomonas
-
ESTs - 06/07/11 (requires password)
 

Updated June 5th, 2009

[Home]