Merchant Lab Research Resources
 
Merchant Lab Research Resources
Antibodies
- Antibodies vs 27March08.xls - updated March 2008 (requires password
)
Archived Protocols
- Chlamy Chloroplast DNA Protocol
- Chloroplast DNA Prep Protocol
- DNA Preparation from Chlamydomonas Reinhardtii
- Pronase Method for Making DNA From C.r.  
Chemical Inventory - see computer in 5069

Enzymes
- Alphabetical List of NEB Enzymes
- Cleavage Near End of Linear DNA
- Double Digests Buffer Compatibility
- Heat Inactivation
- NEBcutter
- Restriction Enzymes Available

- Setting up a Reaction
- Survival in a Reaction

Facilities Management - report a UCLA facilities problem

Freezer Contents
- Inventory for Chest Freezer - November 2007
- Template for Inventory

Group Meetings Schedule - updated May 2008

Journal Club Papers
- Leichert and Jakob, 2004
- Yamasaki et al. 2007
- Wilson et al. 2007

- Peiter et al. 2007

- Surzycki et al. 2007
- Yoshimoto et al. 2007
- Shao et al. 2007
- Quan et al. 2007

Journal Club Suggestions
-
Hicks et al. 2007
- Ojha and Hatfull, 2007
- Gould et al. 2007
- Miras et al. 2007
- Kimata et al. 2007
- Kobayashi et al. 2007
- Meyer et al. 2007
- Havaux et al. 2007
- Cheng et al. 2007
- Wang et al. 2007
- Wiedemann et al. 2007
- Narsai et al. 2007
- Mukherjee et al. 2007
- Bihlmaier et al. 2007

- Rust, et al. 2007
- Ishihara et al. 2007
- Lee et al. 2007
- Chae et al. 2007
- Shimada et al. 2007
- Delneri et al. 2007
- Camargo et al. 2007
- Eveland et al. 2007
- Mills et al. 2007
- DalCorso et al. 2007
- Wagner et al. 2007
- Ridge et al. 2008
- Imlay 2008
- Regulski et al. 2008
- Horan et al. 2008

Articles of Interest (requires password)
- Mineral Nutrition from Lambers et al. (Part 1) (Part 2) (Part 3) (Part 4)
- HHMI Project Management for Young Investigators

Lab Manual
- Add database to Mascot server
- Agencourt Instructions Generic
- arg2 vs. arg7 - Courtesy of Lib Harris
- Amino acid analysis at UC Davis
- Brij lysis plasmid prep purified by CsCl gradients
- Cell and size counts by Coulter counter
- Chlamy growth medium
- Chlamy strains storage protocol by liquid N2 freezing (from Paris group via Olivier)
- Chlamydomonas DNA Midiprep
- Chlorophyll assay
     - Porra et al 2002
     - Porra et al 1989

- Chlorophyll Measurement of Cells in a Culture
- CW Determination
- Deb Berthold's suggestions for protein expression in E. coli
- Dynamics of Microbial Growth
- EMBO Course Protocols
- Endnote X1
- Formaldehyde Gel Electrophoresis of RNA & RNA Blot Hybridization
- Freezing protocol (from Umen lab)
- Home-made ECL Reagents
- Lowry Assay
- Merchant Lab qPCR Protocol (requires password)
- Metal content of chlamy cells and chlamy medium
- Methods of Possible Interest
    - Chl and carotenoids
- Native Gel "Angel" Stock Solutions
- Negative Protein Stain
- Oligolabelling Procedure
    - Info on oligolabelling
    - Optimization
    - Optimization - template concentration & size
    - Protocol
    -
Radiochemical Degradation of Nucleic Acids by 32-Phosphorus
    - Schematic
- Operations Manuals
    - O2 Electrode Preparation and Maintenance
- Optimum use of NBS Universal Platform
- Preparation of Dialysis Tubing
- Preparation of Iron-Deficient Medium
- Purification of Taq (requires password)
- Pipette Washing Instructions
- Radiation Safety Protocols and Procedures (requires password)
- Rapid Isolation of Plasmid DNA - Midi Prep
- RNA isolation from Chlamydomonas
- Springer Protocols
- TAP medium preparation from SM files (original from Togasaki)
- Trypsin in gel digestion

- What is Microbiology?
- Zygote DNA Prep (from Jim Umen)

Lab Responsibilities - updated July 2007
Molecular Weight Markers (requires password)
 - Protein ladders
 - DNA ladders
 - RNA ladders
Homologies, Orthologs and Paralogs
- Orthologs and paralogs - we need to get it right
- Homology - a personal view on some of the problems
- “Homology” in proteins and nucleic acids: A terminology muddle and a way out of it
- Phylogeny for the faint of heart: a tutorial
Online Courses
- Animal Subjects
- Sexual Harrassmment
- Workplace Ethics

Personnel (requires password)

Places to Recycle
Purchase Order List
Replicates & Statistics
- Basic Statistical Considerations in Virological Experiments
- Error Bars
- Experimental Design for Yeast Genomic Expression Analyses

- Sample Size
- Statistical analysis of real time PCR data
- The Power of Replicates
Research Projects
Strain List
- Bacteria
- Yeast
- Chlamydomonas
-
ESTs - 06/07/11 (requires password)
 

Updated April 2008

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